Single-Nucleotide Gene Polymorphisms Involving Cell Death Pathways: A Study of Chinese Patients with Lumbar Disc Herniation

Abstract

Objective: To evaluate the effect of polymorphisms of death pathway genes FAS and FASL on the risk of developing lumbar disc herniation (LDH) in a Northern Chinese population.

Background Data: The FAS receptor–ligand system plays a key role to regulate apoptosis of cell. There is evidence that the apoptosis-mediated FAS receptor–ligand system is involved in the pathogenesis of disc degeneration. Some research considered single-nucleotide polymorphisms of FAS-1377G/A, FAS-670A/G, FASL-844T/C, and FASL INV2nt-124A/G may increase the risk of developing cancer. We therefore assess these four single-nucleotide polymorphisms as candidate susceptibility for LDH.

Methods: A total of 475 patients with LDH and 533 control subjects were selected. Genotypes were determined by polymerase chain reaction-based restriction fragment length polymorphism and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Associations with the risk of LDH were estimated by logistic regression model.

Results: Significant differences were found in genotypic distributions between cases and controls for FASL-844T/C, but not for other three polymorphisms. When compared with CC genotype, subjects with the TT genotype had a higher risk to develop LDH (odds ratio = 3.12; 95% confidence interval: 1.73–5.40). Moreover, an association was found between this genotype of FASL-844TT and more severe grades of disc degeneration. We observed statistically significant interactions between polymorphisms of FASL-844T/C and lumbar load, tobacco smoking, and age.

Conclusion: Genetic polymorphisms of FASL-844T/C may be associated with an increased risk of developing disc degeneration and LDH.

• lumbar disc herniation
• disc degeneration
• apoptosis
• FAS/FASL polymorphisms
• interactions
• case–control study