Immunochemistry of 7 S Domain of Type IV Collagen

Saryu N. Dixit Lakeside V.A. Medical Center and Department of Oral Biology, Northwestern University, Chicago, IL, 60611; Department of Medicine, University of Tennessee, Center for Health Sciences Memphis, TN, 38103

William Robinson Lakeside V.A. Medical Center and Department of Oral Biology, Northwestern University, Chicago, IL, 60611; Department of Medicine, University of Tennessee, Center for Health Sciences Memphis, TN, 38103

Purnima Dixit Lakeside V.A. Medical Center and Department of Oral Biology, Northwestern University, Chicago, IL, 60611; Department of Medicine, University of Tennessee, Center for Health Sciences Memphis, TN, 38103

Andrew H. Kang Lakeside V.A. Medical Center and Department of Oral Biology, Northwestern University, Chicago, IL, 60611; Department of Medicine, University of Tennessee, Center for Health Sciences Memphis, TN, 38103

Abstract

The 7 S domain of type IV collagen was isolated from various basement membranes by pepsin and bacterial collagenase digestion. The antibodies were raised in rabbits against human placental 7 S and bovine cortex 7 S. The antisera were studied for their binding to 7 S samples. The results showed a lack of tissue specificity to 7 S from the same species. In contrast no cross-reactivity was found between 7 S from different species. A set of peptides from 7 S were also prepared and tested for their binding to antiserum, showing retention of some antigenic sites.

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Immunochemistry of 7 S Domain of Type IV Collagen

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Immunochemistry of 7 S Domain of Type IV Collagen

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