Mechanism of crosslinking of proteins by glutaraldehyde III. Reaction with collagen in tissues

David T. Cheung Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Biochemistry, 2400 S. Flower Street, Los Angeles, CA, 90007; Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Medicine, 2400 S. Flower Street, Los Angeles, CA, 90007; Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Orthopaedics, 2400 S. Flower Street, Los Angeles, CA, 90007

Natasha Perelman Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Biochemistry, 2400 S. Flower Street, Los Angeles, CA, 90007; Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Medicine, 2400 S. Flower Street, Los Angeles, CA, 90007; Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Orthopaedics, 2400 S. Flower Street, Los Angeles, CA, 90007

Ellen C. Ko Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Biochemistry, 2400 S. Flower Street, Los Angeles, CA, 90007; Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Medicine, 2400 S. Flower Street, Los Angeles, CA, 90007; Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Orthopaedics, 2400 S. Flower Street, Los Angeles, CA, 90007

Marcel e. Nimni Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Biochemistry, 2400 S. Flower Street, Los Angeles, CA, 90007; Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Medicine, 2400 S. Flower Street, Los Angeles, CA, 90007; Bone and Connective Tissue Research Laboratory, Orthopaedic Hospital/University of Southern California Medical School, Department of Orthopaedics, 2400 S. Flower Street, Los Angeles, CA, 90007

Abstract

Bovine pericardium, a dense collagenous connective tissue, was crosslinked with glutaraldehyde using different modalities of fixation. The degree of crosslinking was evaluated as a function of the ability of CNBr and pronase to solubilize collagen. Our results suggest that glutaraldehyde fixes primarily the surface of the fibers and creates a polymeric network which hinders the further crosslinking of the interstitium of the fiber. When a low concentration of glutaraldehyde was used, a slow time-dependent crosslinking process was observed. This slow process is maintained over a long period of time, greatly beyond that required for the actual penetration of glutaraldehyde to occur.

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Mechanism of crosslinking of proteins by glutaraldehyde III. Reaction with collagen in tissues

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Mechanism of crosslinking of proteins by glutaraldehyde III. Reaction with collagen in tissues

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