Leukotriene B₄ Production by Peripheral Blood Neutrophils in Rheumatoid Arthritis

Abstract

Leukotriene B₄ (LTB₄) is an activator of white blood cells (WBC) and it has been suggested that its inhibition may be useful in rheumatoid arthritis (RA). Its production by peripheral WBC has not yet been investigated. We measured LTB₄ production in 105 patients with RA and compared it with 59 matched controls. C-reactive protein (CRP) and ESR were measured in 90 patients and correlated with LTB₄ values. Ten millilitres of blood were drawn. Separation was undertaken to obtain polymorphonuclear leukocytes (PMN) which were stimulated with calcium ionophore, and the supernatant was frozen for radioimmunoassay of LTB₄. Results show that RA patients produce significantly higher levels of LTB₄. It has been suggested that blockage of the cyclo-oxygenase enzyme by non-steroid antiinflammatory drugs (NSAID) leads to increased production of LT via the lipoxygenase enzyme. Twenty-one patients not taking NSAID were compared with 84 on therapy. There was no significant difference. A linear regression was used to obtain Pearson's correlation coefficients. With LTB₄ and CRP, r=0.3 (p<0.003). With LTB₄ and ESR, r=0.25 (p<0.02). Low but significant correlations with CRP and ESR were obtained.

Key Words:

• leukotriene B₄
• rheumatoid arthritis
• polymorphonuclear leukocytes
• erythrocyte sedimentation rate
• C-reactive protein