Determination of Human Hemoglobin Derivatives

Atef M. M. AttiaBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt Correspondence[email protected]

Fatma A. A. IbrahimBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt

Noha A. Abd El-LatifBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt

Samir W. AzizBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt

Sherif A. Abdelmottaleb MoussaBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt ;Department of Physics, College of Science, Al-Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi Arabia

Mohsen S. ElalfyPediatric Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Abstract

The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727–0.370%), metHb% (0.43–1.0%), HbCO% (0.4–1.52%) and oxyHb% (97.06–98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608–15.777 g/dL). The method is highly sensitive, accurate and reproducible.

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Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

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Determination of Human Hemoglobin Derivatives

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Determination of Human Hemoglobin Derivatives

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