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Abstract
A double antibody assay for the determination [N-(4-amino)phenyl] aminophenyl iminodiacetic acid (I) and a number of its derivatives in serum has been developed. The same antiserum and tracer were used for the determination of the serum levels of derivatives of (I). The minimum detectable concentration of I that was detectable was 6 ng/ml. The precision error was less than 1% and the accuracy as indicated by the recovery ratios was satisfactory for all derivatives of I.