Preparation and Evaluation of Eudragit E Microspheres Containing Bacampicillin

Abstract

Microspheres with bacampicillin were prepared by the solvent evaporation method using systems methanol, acetone and methyl acetate/liquid paraffin and Eudragit E as polymer. Sieve analysis showed that the particle size of the microspheres follows log - normal distribution with average size of 123, 206 and 300 μm, respectively. Scanning electron microscopy was used to prove that all chosen systems provided the particles of regular spherical shape without aggregation

HPLC method was developed for testing drug content, drug stability and dissolution. The results of HPLC analysis showed the exisistence of degradation products of bacampicillin in microspheres prepared by the use of all three solvents. The degree of degradation was the lowest in the case of methyl acetate. The experimental values of dissolution profiles fit well to 0. order and combined 0. and t^½ order. The comparison of dissolution profiles of microspheres and bacampicillin itself shows that microspheres produce retard effect and therefore bacampicillin is not expected to be released in saliva after peroral administration of microspheres