Determination of Hydralazine Hydrochloride and Hydrochlorothiazide in Dosage Forms by High Performance Liquid Chromatography

Abstract

A high performance liquid chr0matOgraphiC method is presented for the simultaneous determination of hydralazine hydrochloride and hydrochlorothiazide in combination dosage forms. ccmpounds are chromatographed on a radialpak cyanopropylsilane cartridge with a mixture of methanol, water and dibutylamine phosphate as mobile phase and W detection at 254 run. hydralazine hydrochloride and hydrochlorothiazide showed linear detector responses over a range of 50-150% of label claim with correlation coefficients of 0.999. Assay recoveries (n = 5) were found to contain an average of 98.9% hydrochloride and 98'.8% 2 1.1 of hydrochlorothiazide. proposed method showed excellent resolution and reproducibility. It will be helpful in routine quality control analysis of such combination dosage forms.