Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems
January 2010, Vol. 28, No. 1
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Pages 7-12
Leda Roncoroni,1,2,3 Luca Elli,2 Maria Teresa Bardella,2,3 Elena Dogliotti,1 Maria Grazia Grimoldi,4 Silvia Viaggi,5,6 Eugenio Erba,7 Annalisa Zunino,5 and Luisa Doneda,3
Department of Biology and Genetic for Health Sciences, University of Milan, Via Viotti, Milan, Italy,1
Center for Prevention and Diagnosis of Celiac Disease, Fondazione IRCCS Policlinico, Mangiagalli e Regina Elena, Via F. Sforza, Milan, Italy,2
Department of Medical Sciences, University of Milan, Via Viotti, Milan, Italy,3
Department of Medicine, Surgery and Dental Sciences, Pathology Unit, University of Milan, Via Rudinì, Milan, Italy,4
National Cancer Research Institute, Largo Benzi Rosanna, Genoa, Italy,5
Department of Biology, University of Genoa, Via Balbi, Genoa, Italy,6
Flow Cytometry Unit, Department of Oncology, Mario Negri Institute, Via La Masa, Milan, Italy,7
ABSTRACT
The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.
