Epidermal Growth Factor Induces Cell Death in the Absence of Overexpressed Epidermal Growth Factor Receptor and ErbB2 in Various Human Cancer Cell Lines

ABSTRACT

Epidermal growth factor (EGF) stimulates apoptosis in tumor cells depending on the expression levels of EGF receptor and Her2. We examined the protein levels in 22 different cancer cell lines and assessed the responses to EGF. EGF-induced cytotoxicity was not correlated with the levels of either EGFR or Her2. Fourteen cell lines exhibited decreased cell proliferation, whereas 293T cells did not display any noticeable changes and degraded transiently expressed EGFR following EGF treatment. EGF treatment resulted in significant tumor growth inhibition in some xenografts. Our data indicate that exogenous EGF treatment leads to growth inhibition rather than inducing tumor cell proliferation.

Keywords: :

• Epidermal growth factor (EGF)
• Epidermal growth factor receptor (EGFR)
• ErbB2
• Apoptosis

ACKNOWLEDGMENTS

This work is supported by the National Research Foundation Korea (NRF) grant funded by the Korean Government (2007-E00111 and 2009-0081016), and a grant of the Korea Health 21 R&D Project, Ministry of Health, Welfare and Family Affairs, Republic of Korea (A062254).

Declaration of interest: The authors report no conflict of interest. The authors alone are responsible for the content and writing of this paper.