Peroxiredoxin 6 Overexpression Attenuates Cisplatin-Induced Apoptosis in Human Ovarian Cancer Cells

Jhang Ho PakAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan, Korea, and Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, KoreaCorrespondence[email protected]

Won Ho ChoiAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan, Korea, and Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

Hye Mi LeeAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan, Korea, and Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

Won-Duk JooAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan, Korea, and Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

Jong-Hyeok KimAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan, Korea, and Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

Young-Tak KimAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan, Korea, and Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

Yong-Man KimAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan, Korea, and Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

Joo-Hyun NamAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan, Korea, and Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

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Abstract

We examined the involvement of peroxiredoxin 6 (Prdx 6) in providing chemoprotection against cisplatin cytotoxicity in SKOV-3 ovarian cancer cells. Treatment of SKOV-3 cells with cisplatin-induced cytotoxicity that was associated with increased accumulation of intracellular reactive oxygen species (ROS) and apoptosis mediated by proteolytically activated caspase 3 and 9. Overexpression of Prdx 6 protein or exposure to N-acetylcysteine (NAC) reversed the apoptotic effect of cisplatin by reducing ROS levels and suppressing the caspase signaling pathway. These results indicate that targeting Prdx 6 may sensitize cancer cells to ROS-producing therapeutic treatments, such as anticancer drugs and radiation.

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ACKNOWLEDGMENT

This study was supported by a grant (R01-2006-000-11189-0) from the Korea Science and Engineering Foundation (KOSEF) and by a grant (2009-018) from the Asan Institute for Life Sciences, Seoul, Korea. We thank Ju Hyun Moon for the preparation of figures.

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Peroxiredoxin 6 Overexpression Attenuates Cisplatin-Induced Apoptosis in Human Ovarian Cancer Cells

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Peroxiredoxin 6 Overexpression Attenuates Cisplatin-Induced Apoptosis in Human Ovarian Cancer Cells

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