Thrombin Expression in Prostate: A Novel Finding

Manish KohliDepartment of Oncology, Mayo Clinic, Rochester, Minnesota, USA, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, USA, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA, Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA, Department of Urology, SUNY Upstate Medical University, Syracuse, New York, USA and Department of Urology & Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USACorrespondence[email protected]

Karin WilliamsDepartment of Oncology, Mayo Clinic, Rochester, Minnesota, USA, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, USA, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA, Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA, Department of Urology, SUNY Upstate Medical University, Syracuse, New York, USA and Department of Urology & Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA

Jorge L. YaoDepartment of Oncology, Mayo Clinic, Rochester, Minnesota, USA, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, USA, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA, Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA, Department of Urology, SUNY Upstate Medical University, Syracuse, New York, USA and Department of Urology & Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA

Richard A. DennisDepartment of Oncology, Mayo Clinic, Rochester, Minnesota, USA, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, USA, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA, Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA, Department of Urology, SUNY Upstate Medical University, Syracuse, New York, USA and Department of Urology & Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA

Jiaoti HuangDepartment of Oncology, Mayo Clinic, Rochester, Minnesota, USA, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, USA, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA, Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA, Department of Urology, SUNY Upstate Medical University, Syracuse, New York, USA and Department of Urology & Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA

Jay ReederDepartment of Oncology, Mayo Clinic, Rochester, Minnesota, USA, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, USA, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA, Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA, Department of Urology, SUNY Upstate Medical University, Syracuse, New York, USA and Department of Urology & Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA

William A. RickeDepartment of Oncology, Mayo Clinic, Rochester, Minnesota, USA, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, USA, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA, Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA, Department of Urology, SUNY Upstate Medical University, Syracuse, New York, USA and Department of Urology & Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA

show all

Abstract

Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.

Keywords: :

ACKNOWLEDGMENTS

The study was supported by Departmental funds from Medicine and Urology, University of Rochester, NCI grant no. CA091956 (Prostate SPORE Awarded to Mayo Clinic), and Funding source, CA123199 (WAR).

The authors are grateful to Professor Charles Francis, MD, Director, Coagulation Lab, University of Rochester, NY, for editorial comments and suggestion with manuscript preparation.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Thrombin Expression in Prostate: A Novel Finding

Information for

Open access

Opportunities

Help and information

Thrombin Expression in Prostate: A Novel Finding

Abstract

ACKNOWLEDGMENTS

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature