EGFR Gene Overexpression Retained in an Invasive Xenograft Model by Solid Orthotopic Transplantation of Human Glioblastoma Multiforme Into Nude Mice

Abstract

Orthotopic xenograft animal model from human glioblastoma multiforme (GBM) cell lines often do not recapitulate an extremely important aspect of invasive growth and epidermal growth factor receptor (EGFR) gene overexpression of human GBM. We developed an orthotopic xenograft model by solid transplantation of human GBM into the brain of nude mouse. The orthotopic xenografts sharing the same histopathological features with their original human GBMs were highly invasive and retained the overexpression of EGFR gene. The murine orthotopic GBM models constitute a valuable in vivo system for preclinical studies to test novel therapies for human GBM.

Keywords: :

• Glioblastoma multiforme
• Orthotopic xenograft
• Nude mice

ACKNOWLEDGMENTS

Supported by City Health Administration of Xiamen grant 3502z20089013. We are grateful to Jing Jing Liu, M.D., Hui Qin Zhuo, M.D., and Qin Yao, M.M., in the Pathology Department and Laboratory of Molecular Biology of Affiliated Zhongshan Hospital, Xiamen University, for providing the technical assistance.