Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach

Jingjing LvInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China,;The Second Military Medical University, Shanghai, China,

Naijun FanInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China,

Yangkun WangDepartment of Pathology, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China

Xiuli WangInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China,

Chunfang GaoInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China,Correspondence[email protected]

Abstract

Liquid chromatograph-mass spectrometer (LC/MS) based labeled with isobaric mass tags for relative and absolute quantitation (iTRAQ) analyses were performed to identify differentially expressed proteins from normal and cancerous human colorectal tissues. Around 802 proteins were identified, 68 proteins of which were defined as differentially expressed proteins. Bioinformatics analysis indicated that these differentially expressed proteins correlated with several specific cellular processes and pathways which have relationships with pathological changes of colorectal cancer (CRC). EHD2 were selected to verify its expression patterns and localization using western blotting and immunohistochemistry respectively. LC/MS-based iTRAQ proteomic approach would provide new information about the character of CRC.

Keywords: :

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach

Information for

Open access

Opportunities

Help and information

Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature