PHASE AND PERIOD RESPONSES OF THE JERBOA JACULUS ORIENTALIS TO SHORT LIGHT PULSES

Abstract

The phase and period responses to short light pulses were studied in the jerboa, a seasonal, hibernating, nocturnal rodent from the Atlas region in Morocco. The jerboa, which is a saltatory species, showed precise activity onsets and offsets under a light-dark (LD) cycle using infrared captors to record locomotor activity. When released into constant darkness (DD), the majority of animals showed a circadian period (τ) <24 h (mean τ = 23.89 ± 0.13 h) and a lengthening of the activity span, α. Animals were subsequently exposed to up to eight 15-min light pulses, each separated by at least 2 wks, for up to 160 days in DD. During this span, most individuals maintained robust circadian rhythmicity, with clearly defined activity onsets and offsets, similar levels of total activity, duration of α, and percent activity occurring during the subjective night. The phase response curve (PRC) is typical of other nocturnal rodents, with light eliciting delays during late subjective day and early subjective night (CT8–CT19) and advances during late subjective night to early subjective day (CT19–CT2). A dead zone, when light had no effect on phase, is observed during mid-subjective day (CT3–CT8). A few individuals showed large (>9 h) Type 0 phase resetting near the singularity region (CT19) that resulted in a complete phase reversal, but otherwise displayed normal phase-shifting responses at other CT times. The τ response curve showed a decrease in period from early to late subjective night with increases at other times, but these changes were small (maximum <9 min) and highly variable. There was a distinct tendency for animals that had an initial short τ in DD to conserve a short τ during the series of light pulses and, inversely, for animals with long τ to conserve a long τ. This suggests possible constraints on the plasticity of variation of τ in relation to the endogenous period of the animal. (Author correspondence: [email protected])

Keywords:

• Circadian activity
• Jaculus orientalis
• Light
• Period response curve
• Phase response curve
• Photic entrainment

ACKNOWLEDGMENTS

This work was supported by grants from Rhône-Alpes MIRA, MAE-PHC Volublis (no. 20565ZM), EUCLOCK (EU-FP6 project no. 018741).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.