Food Availability Affects Circadian Clock-Controlled Activity and Zugunruhe in the Night Migratory Male Blackheaded Bunting (Emberiza melanocephala)

Abstract

This study investigated the functional linkage between food availability and activity behavior in the Palaearctic Indian night migratory blackheaded bunting (Emberiza melanocephala) subjected to artificial light-dark (LD) cycles. Two experiments were performed on photosensitive birds. In the first one, birds were exposed to short days (LD 10/14; Experiment 1A), long days (LD 13/11; Experiment 1B), or increasing daylengths (8 to 13 h light/d; Experiment 1C) and presented with food either for the whole or a restricted duration of the light period. In Experiments 1A and 1B, illumination of the light and dark periods or of the dark period, alone, was changed to assess the influence of the light environment on direct and circadian responses to food cycles. In the second experiment, birds were exposed to LD 12/12 or LD 8/16 with food availability overlapping with the light (light and food presence in phase) or dark period (light and food presence in antiphase). Also, birds were subjected to constant dim light (LL_dim) to examine the phase of the activity rhythms under synchronizing influence of the food cycles. Similarly, the presentation of food ad libitum (free food; FF) during an experiment examined the effects of the food-restriction regimes on activity rhythms. A continuous measurement of the activity-rest pattern was done to examine both the circadian and direct effects of the food and LD cycles. Measurement of activity at night enabled assessment of the migratory phenotype, premigratory restlessness, or Zugunruhe. The results show that (i) light masked the food effects if they were present together; (ii) birds had a higher anticipatory activity and food intake during restricted feeding conditions; and (iii) food at night alone reduced both the duration and amount of Zugunruhe as compared to food during the day alone. This suggests that food affects both the daily activity and seasonal Zugunruhe, and food cycles act as a synchronizer of circadian rhythms in the absence of dominant natural environmental synchronizers, such as the light-dark cycle. (Author correspondence: [email protected])

Keywords:

• Bunting
• Circadian rhythm
• Food
• LD cycle
• Migratory
• Zugunruhe

ACKNOWLEDGMENTS

A generous financial support through an IRHPA grant (IR/SO/LU-02/2005) from the Department of Science and Technology, New Delhi, India, is gratefully acknowledged. The experiments reported herein were performed at the University of Lucknow, India, as approved by the Institutional Ethics Committee.

Declaration of Interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.