Induction of Cell-Mediated Immune Responses to Peptide Antigens of P. vivax in Microparticles Using Intranasal Immunization

Abstract

T-cells play a critical role in resistance to malaria, not only because they function as helper cells for an antibody response, but also because they serve as effector cells. Such cellular immunity is directly implicated in protection from sporozoites as well as from blood stage parasites. The aim of this study was to induce cell mediated immune responses to peptide antigens of Plasmodium vivax co-encapsulated with CpG oligodeoxynucleotide (ODN) in microparticles. In the present study, we have investigated the immunomodulatory effects of two CpG adjuvants, CpG 1826 and CpG 2006 to the five peptide antigens of Plasmodium vivax derived from circumsporozoite protein, merozoite surface protein-1, apical membrane antigen-1 and gametocyte surface antigen (Pvs24) in microparticle delivery. The T-cell proliferation response study of the cells collected from spleen, lamina propria and peyer's patches showed significantly high (p<0.001) stimulation index when primed with peptide antigens in microparticles co-encapsulating CpG ODN adjuvant as compared to peptide alone primed mice. The cytokine measurement profile of IFN-γ, TNF-α, IL-2, IL-4 and IL-10 in culture supernatants of cells primed with peptide antigens in microparticles co-encapsulating CpG ODN showed higher levels of IFN- γ followed by TNF-α and IL-2, with relatively low levels of IL-4 and IL-10.

Keywords:

• CD4+ cells
• CpG
• Cytokines
• Intranasal immunization
• Microparticles
• Peptide

ACKNOWLEDGMENTS

The authors are grateful to Indian Council of Medical Research, New Delhi for financial help and fellowship to Mr. Ajaz Ahmad Bhat.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.