Preparation of Microspheres of Poly(Glycidyl Methacrylate) and its Derivatives as Carriers for Immobilized Proteins

Abstract

Microspheres of poly(glycidyl methacrylate) and its derivatives have been prepared. The diameter can be varied in the range from 0.5 to 5μm. Proteins were immobilized on the microspheres by various processes. The maximum amount of covalently immobilized γ-globulin on the microspheres was found to be about 6mg/m². It was shown that dyed microspheres with a diameter of 2 - 4μm are applicable to immunological agglutination tests on a microplate as carrier particles substituting for red blood cells.