Cytokine Profile and Natural Killer Cell Activity in Listeria Monocytogenes Infected Mice Treated Orally with Petiveria Alliacea Extract

Abstract

In this work, we investigated the effects of Petiveria alliacea extract on the production of Th1-type and Th2-type cytokines and on NK cells activity in normal and Listeria monocytogenes infected mice. Our results demonstrated that in normal/non-infected mice P. alliacea administration led to increased levels of Interleukin-2 (IL-2). The infection alone enhanced INF-γ levels and NK cell activity at 48 and 72 hours of infection. The treatment with five consecutive doses of 1000mg/kg/day of P. alliacea extract, given previously to infection, led to further increases in IL-2 levels, in relation to normal/non-infected/P. alliacea treated controls, and in INF-γ levels at 72h of infection, compared to infected mice. On the other hand, the production of IL-4 and IL-10 were not altered either by the infection or by the treatment with P. alliacea extract. NK cells activity increased at 48h and 72h following the inoculation of the bacteria. When mice were treated with P. alliacea previously to infection, NK activity was higher than that observed at 48h, 72h and 120h of infection in the infected animal. Based on these findings we suggest that P. alliacea up-regulates anti-bacterial immune response by enhancing both Th1 function and the activity of NK cells.

Keywords:

• Listeria monocytogenes
• Petiveria alliacea
• cytokines
• IL-2
• IL-4
• EFN-γ
• IL-10
• NK cells