Exploring the potential of Nelumbo nucifera rhizome on membrane stabilization, mast cell protection, nitric oxide synthesis, and expression of costimulatory molecules

Abstract

Immunomodulatory activity of Nelumbo nucifera rhizome was evaluated for its standardized extract (NNRE) with respect to betulinic acid. Various key parameters including erythrocyte membrane stabilization, inhibition of histamine release, reduction in nitric oxide production and depletion of expression of costimulatory molecules of macrophages were estimated. The result displayed that NNRE stabilized erythrocyte membrane significantly at 10 (42.05%) and 100 μg/mL (44.31%). Although considering the protection of mast cells from degranulation, NNRE showed 38.66% (100 μg/mL) and 69.66% (10 μg/mL) degranulation against compound 48/80 (C 48/80). NNRE at 1 and 5 μg/mL inhibited lipopolysaccharide (LPS)-induced activation of macrophages by decreasing the expression of costimulatory molecules. Expression of CD40, CD80, and CD86 by NNRE was seen significantly at 5 μg/mL compared to LPS-treated group. The extracts also inhibited the nitrite concentration at 1 and 5 μg/mL compared to LPS-treated group.

Keywords:

• Nelumbo nucifera rhizome
• mast cells
• costimulatory molecules
• NO production
• betulinic acid