Bone marrow-derived mesenchymal stem cells attenuate phosgene-induced acute lung injury in rats

Junfeng ChenCenter of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China, ;Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai, China, and

Yiru ShaoCenter of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China, ;Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai, China, and

Guoxiong XuCenter Laboratory, Jinshan Hospital, Fudan University, Shanghai, China

ChitChoon LimCenter of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China, ;Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai, China, and

Jun LiCenter of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China, ;Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai, China, and

Daojian XuCenter of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China, ;Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai, China, and

Jie ShenCenter of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China, ;Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai, China, and Correspondence[email protected]

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Abstract

Accidental phosgene exposure could result in acute lung injury (ALI), effective therapy is needed for the patients with phosgene-induced ALI. As a type of cells with therapeutic potential, mesenchymal stem cells (MSCs) have been showed its efficacy in multiple diseases. Here, we assessed the therapeutic potential of MSCs in phosgene-induced ALI and explored the related mechanisms. After isolation and characterization of rat bone marrow MSCs (BMMSCs), we transplanted BMMSCs into the rats exposed to phosgene and observed significant improvement on the lung wet-to-dry ratio and partial oxygen pressure (PaO₂) at 6, 24, 48 h after phosgene exposure. Histological analyses revealed reduced sign of pathological changes in the lungs. Reduced level of pro-inflammatory tumor necrosis factor α and increased level of anti-inflammatory factor interleukin-10 were found in both bronchoalveolar lavage and plasma. Significant increased expression of epithelial cell marker AQP5 and SP-C was also found in the lung tissue. In conclusion, treatment with MSC markedly decreases the severity of phosgene-induced ALI in rats, and these protection effects were closely related to the pulmonary air blood barrier repairment and inflammatory reaction regulation.

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Declaration of interest

This work was funded by National Natural Science Foundation of China (81471850), National Natural Science Foundation of China (81101412), and Science and Technology Commission of Shanghai Municipality (11JC1401900).

Supplementary material available online

Supplementary Figure S1

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Bone marrow-derived mesenchymal stem cells attenuate phosgene-induced acute lung injury in rats

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Bone marrow-derived mesenchymal stem cells attenuate phosgene-induced acute lung injury in rats

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