Chain-specific antibodies for laminin-511

Laura ZamursThe Ludwig Institute for Cancer Research, Melbourne Branch Parkville, VictoriaAustralia;Department of Surgery, University of Melbourne, Royal Parade, Royal Melbourne Hospital VictoriaAustralia

Normand PouliotMetastasis Research Laboratory, Peter MacCallum Cancer Centre, St. Andrews Place East Melbourne, VictoriaAustralia;Sir Peter MacCallum Department of Oncology, University of Melbourne VictoriaAustralia;Pathology Department, University of Melbourne VictoriaAustralia

Nicole KusumaMetastasis Research Laboratory, Peter MacCallum Cancer Centre, St. Andrews Place East Melbourne, VictoriaAustralia;Pathology Department, University of Melbourne VictoriaAustralia

Edouard C. NiceThe Ludwig Institute for Cancer Research, Melbourne Branch Parkville, VictoriaAustralia;Department of Biochemistry and Molecular Biology, Monash University Clayton, VictoriaAustraliaCorrespondence[email protected]
[email protected]

Antony W. BurgessThe Ludwig Institute for Cancer Research, Melbourne Branch Parkville, VictoriaAustralia;Department of Surgery, University of Melbourne, Royal Parade, Royal Melbourne Hospital VictoriaAustralia;The Walter and Eliza Hall Institute of Medical Research Parkville, VictoriaAustraliaCorrespondence[email protected]

Abstract

Mouse monoclonal antibodies (mAbs) that bind to specific chains of laminin-511 (LM-511) have been developed. Antibody 2F12 binds to the LMα5 chain, 3G10 binds to the LMβ1 chain and 3C12 binds to the LMγ1 chain. These antibodies can be used to purify LM-511, to detect LM-511 in cell extracts or to detect the location of LM-511 in tissue by immunohistochemistry. In combination, the antibodies recognize all three chains of LM-511 and combinations of the antibodies can be used to quantitate levels of LM-511 in physiological fluids. One of the antibodies (3G10) is a potent inhibitor of the activity of LM-511 in cell adhesion, spreading and proliferation assays.

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Chain-specific antibodies for laminin-511

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Chain-specific antibodies for laminin-511

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