Rat Brain Capillary Endothelial Cells Express Functional PDGF B-Type Receptors

Anja Smits Ludwig Institute for Cancer Research, Box 595, Biomedical Center and University Hospital, S-75123, Uppsala, Sweden

Monica Hermansson Ludwig Institute for Cancer Research, Box 595, Biomedical Center and University Hospital, S-75123, Uppsala, Sweden

Monica Nister Department of Pathology, University Hospital, Uppsala, Sweden

Irina Karnushina Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden

Carl-Henrik Heldin Ludwig Institute for Cancer Research, Box 595, Biomedical Center and University Hospital, S-75123, Uppsala, Sweden

Bengt Westermark Department of Pathology, University Hospital, Uppsala, Sweden

Keiko Funa Ludwig Institute for Cancer Research, Box 595, Biomedical Center and University Hospital, S-75123, Uppsala, Sweden

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Abstract

Immunohistochemical staining revealed the presence of platelet-derived growth factor (PDGF) B-type receptors on capillaries of normal rat brain. Furthermore, capillary endothelial cells isolated from rat brain and grown in tissue culture bound [¹²⁵I)PDGF-BB but not [¹²⁵I]PDGF-AA, suggesting that they expressed B-type, but not A-type, PDGF receptors. PDGF-BB and PDGF-AB, but not PDGF-AA, also stimulated incorporation of [³H]thymidine into these cells. Thus, rat brain capillary endothelial cells have functional B-type receptors, and thereby differ from endothelial cells derived from large blood vessels, that do not express PDGF receptors. Our data suggest a possible role for PDGF-BB as an angiogenic factor.

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Rat Brain Capillary Endothelial Cells Express Functional PDGF B-Type Receptors

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Rat Brain Capillary Endothelial Cells Express Functional PDGF B-Type Receptors

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