The late effects of radiation on lifespan, lymphocyte proliferation and p53 haplodeficiency in mice

Abstract

Purpose: We investigated the effect of irradiation on the lifespan of eight-week-old mice, the number of lymphocytes in bone marrow and the levels of p53 protein expression in the splenocytes.

Methods and materials: Eight-week-old mice, wild-type p53 (p53^+/+) and heterozygous p53 (p53^+/−), were irradiated with 3 Gy. The cell numbers and cell cycle phases of bone marrow cells were determined by flow cytometry. The splenocyte proliferation was evaluated by a fluorescent cell viability assay. The p53 expression was evaluated by Western blotting.

Results: The lifespan of the irradiated mice was shorter than that of the non-irradiated mice. In irradiated 72-week-old p53^+/+ mice and 56-week-old p53^+/− mice, the number of lymphocytes in bone marrow decreased as compared to that in the non-irradiated mice. In 56-week-old p53^+/− mice, the S- and G2/M-phases of lymphocytes in the irradiated mice were increased compared to that in the non-irradiated mice. The splenocyte proliferation in p53^+/+ mice decreased with age, and the proliferation in the irradiated mice was much lower than that in the non-irradiated mice. In 72-week-old p53^+/+ mice after re-irradiation, the p53 protein expression in the splenocytes of the irradiated mice was delayed as compared to those from the non-irradiated mice.

Conclusion: We suggest that the decrease in the number of lymphocytes in bone marrow and the delayed p53 expression in splenocytes from the irradiated mice are related to the shortened lifespan after irradiation at a young age.

Keywords:

• Irradiation
• lifespan
• lymphocytes
• p53
• delayed mutation

Acknowledgements

This work was supported in part by UOEH grant for Advanced Research (H18-3), UOEH Research Grant for Promotion of Occupational Health from University of Occupational and Environmental Health (#909) and Grants-in-Aid for Scientific Research (C) from Japan Society for the Promotion of Science (#19510061).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.