Abstract
Purpose: Propolis, a product of honeybees, has anti-tumoural, cytotoxic, anti-metastatic and anti-inflammatory properties. The aim of this study was the evaluation of the radiosensitising capacity of Propolis in human head and neck squamous cell carcinoma (HNSCC) cells.
Materials and methods: HNSCC cell lines (FaDu, UT-SCC15, UT-SCC45), fibroblasts (HSF2) and keratinocytes (HaCaT) were treated with Propolis (0–250 μg/ml; 1, 4, 24 h) without and in combination with X-rays (0–6 Gy, single dose). Clonogenic survival, proliferation, apoptosis, expression and phosphorylation of different signalling proteins were determined. Liquid chromatography-mass spectrometry (LC-MS) was performed on Propolis.
Results: Propolis significantly (P < 0.01) reduced cell growth and clonogenic survival in a time- and concentration-dependent manner. Propolis-induced apoptosis and Caspase 3 cleavage, increased phosphorylation of Extracellular signal Regulated Kinase 1/2 (ERK1/2), protein kinase B/Akt1 (Akt1) and Focal adhesion kinase (FAK). While a 1-h Propolis pretreatment was ineffective, a 3-h pretreatment significantly (P < 0.05) radiosensitised FaDu cells. LC-MS analysis identified 14 compounds of Propolis.
Conclusions: Our data show that Propolis exerts cytotoxicity in a concentration- and time-dependent manner. In one out of three HNSCC cell lines, Propolis also caused an enhancement of radiosensitivity. Future studies on Propolis will shed further light on its potential as an adjuvant to radiotherapy.
Acknowledgements
The authors are indebted to M. Baumann (Dresden University of Technology, Germany), R. Grenman (Turku University Central Hospital, Finland) and H. P. Rodemann (University Tübingen, Germany) for kindly providing cell lines. The research and authors were in part supported by a grant from the Federal Ministry of Education and Research, Germany (BMBF Contract 03ZIK041).
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.