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Abstract
The temporomandibular joint (TMJ) formalin test was used to evaluate the effects of acute restraint stress on the nociceptive behavioral responses of female rats during proestrus and estrus phases of the estrous cycle. Rats were subjected to one session of restraint stress (15, 30 min or 1 h). They were then either immediately killed to allow the collection of blood for hormonal radioimmunoassay determinations or subjected to TMJ formalin test to evaluate nociception. All stress protocols significantly raised the plasma concentrations of corticosterone. The performance of rats subjected to 15 and 30 min of restraint stress was similar to that of control rats, whereas rats that were stressed for 1 h showed a decrease in nociceptive responses, during both proestrus and estrus phases. The stress-induced analgesia (SIA) was greater in the proestrus phase. To evaluate the role of κ-opioid receptors, the selective receptor κ-opioid antagonist nor-binaltorphimine (nor-BNI; 200 μg or saline) was injected into the TMJ 24 h prior to the 1 h stress period and the TMJ formalin test. The local administration of nor-BNI partially reversed the SIA during the proestrus phase. These findings suggest that (1) acute stress for 1 h can produce analgesia both during proestrus and estrus phases; this effect is greater during the proestrus phase and (2) κ-opioid receptor activation is involved in the SIA observed in the proestrus phase.
Acknowledgements
The authors thank Carlos Alberto Feliciano for technical assistance and Professor Margaret de Castro (FMRP-USP) for radioimmunoassay assistance. This work was supported by CNPq, Brazil.
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.