P450 monooxygenases (P450ome) of the model white rot fungus Phanerochaete chrysosporium

Abstract

Phanerochaete chrysosporium, the model white rot fungus, has been the focus of research for the past about four decades for understanding the mechanisms and processes of biodegradation of the natural aromatic polymer lignin and a broad range of environmental toxic chemicals. The ability to degrade this vast array of xenobiotic compounds was originally attributed to its lignin-degrading enzyme system, mainly the extracellular peroxidases. However, subsequent physiological, biochemical, and/or genetic studies by us and others identified the involvement of a peroxidase-independent oxidoreductase system, the cytochrome P450 monooxygenase system. The whole genome sequence revealed an extraordinarily large P450 contingent (P450ome) with an estimated 149 P450s in this organism. This review focuses on the current status of understanding on the P450 monooxygenase system of P. chrysosproium in terms of pre-genomic and post-genomic identification, structural and evolutionary analysis, transcriptional regulation, redox partners, and functional characterization for its biodegradative potential. Future research on this catalytically diverse oxidoreductase enzyme system and its major role as a newly emerged player in xenobiotic metabolism/degradation is discussed.

Keywords::

• Cytochrome P450 monooxygenase
• white-rot fungus
• biodegradation
• xenobiotics

Acknowledgements

Authors’ original work reported in this article and the compilation efforts were supported by the NIH’s National Institute of Environmental Health Science (NIEHS) grants R01ES10210 and R01ES015543 to JSY.

Declaration of interest

The authors have no declaration of interest to report.