Short Communication: Sequencing and Chromosomal Assignment of the Rat Endothelial-derived Lipase Gene (Lipg)

Anita C.M. Bonné Department of Laboratory Animal Science Veterinary Faculty, Utrecht University, P.O. Box 80166, 3508 TD, Utrecht, The NetherlandsCorrespondence[email protected]

Maria G. Den Bieman Department of Laboratory Animal Science Veterinary Faculty, Utrecht University, P.O. Box 80166, 3508 TD, Utrecht, The Netherlands

Hein A. Van Lith Department of Laboratory Animal Science Veterinary Faculty, Utrecht University, P.O. Box 80166, 3508 TD, Utrecht, The Netherlands

Bert F.M. Van Zutphen Department of Laboratory Animal Science Veterinary Faculty, Utrecht University, P.O. Box 80166, 3508 TD, Utrecht, The Netherlands

Abstract

Part of the nucleotide sequence of the Lipg gene in the rat was established using primers based on the mRNA sequence described in the mouse. The rat intron sequence served as a template for designing primers for the specific amplification of rat Lipg. A rat-hamster radiation hybrid (RH) panel was used for chromosomal assignment of the rat Lipg gene. The Lipg gene was found to be located on rat chromosome 18 in the vicinity of the marker D18Mitll; a region reported to be homologous with both human and mouse chromosome 18.

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Short Communication: Sequencing and Chromosomal Assignment of the Rat Endothelial-derived Lipase Gene (Lipg)

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Short Communication: Sequencing and Chromosomal Assignment of the Rat Endothelial-derived Lipase Gene (Lipg)

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