DNA sequence characterization of the G gene region of bacteriophage Mu

Abstract

The nucleotide sequence of a 1.2 kb region of bacteriophage Mu DNA was determined. This region contains the 3′ end of the F gene, the complete G gene, and the 5′ end of the I gene, all late genes involved in Mu virion morphogenesis. identity of the G gene open reading frame was confirmed by sequencing four Gam mutations. The G open reading frame is predicted to encode proteins of 16.7 or 17.2 kDa, depending on which of two possible start codons are used to initiate translation. Four new nuB mutations in the DNA gyrase-binding site between the G and I genes were also sequenced and found to be identical to the nuB 103 mutation sequenced previously.

Key Words:

• DNA sequence analysis
• Cam mutations
• nuB mutations
• phage Mu late genes
• virion morphogenesis proteins