Detection of BCR-ABL Transcripts by Multiplex and Nested PCR in Different Haematological Disorders

Abstract

Single-step Multiplex RT-PCR was used as a rapid and highly sensitive method for screening patients with myeloproliferative conditions and ALL for the presence of underlying BCR-ABL gene fusions. Positive and negative results obtained with the multiplex assay were subsequently confirmed by nested PCR. We studied 21 patients for detecting the presence of b3a2, b2a2 and ela2 BCR-ABL transcripts at diagnosis and following treatment with different therapeutical procedures. These studies allowed the molecular characterisation of patients with different haematological disorders and for demonstrating BCR-ABL transcripts in Ph⁻ CML. In a Ph⁺ CML patient, a switch of isoforms was detected after bone marrow transplantation and infusion with donor lymphocytes, implying substitution of ela2 for b3a2 coexisting with a myeloid/lymphoid biphenotypic profile. In ALL, one Ph⁺ patient showed coexpression of ela2 and b2a2 at diagnosis followed by persistence of e1a2 after bone marrow transplantation. Our results were compared to previous findings in the literature on molecular diagnosis of leukaemias.

Key Words:

• Chronic Myeloid Leukaemia
• multiplex RT-PCR
• BCR-ABL