Expression patterns of CD200 and CD148 in leukemic B-cell chronic lymphoproliferative disorders and their potential value in differential diagnosis

Abstract

Different combinations of biomarkers analyzed by flow cytometry are critical for the accurate diagnosis of leukemic B-cell chronic lymphoproliferative disorders (B-CLPDs). We investigated CD200 and CD148 expression patterns of blood or bone marrow from 374 cases of B-CLPD by multicolor flow cytometry. Our results showed that CD200 and CD148 expression patterns distinguished different types of B-CLPD. CD200 mean fluorescence intensity (MFI) or CD148 MFI had a high sensitivity and specificity to differentiate mantle cell lymphoma (MCL) from chronic lymphocytic leukemia (CLL). Furthermore, CD148 MFI/CD200 MFI ratio > 4.79 produced a specificity of 94.46% (95% confidence interval [CI]: 91.04–96.87%) and a sensitivity of 100% (95% CI: 88.78–100.0%) in establishing the diagnosis of MCL in differential diagnosis between MCL and CLL. We therefore conclude that the combination of CD200 and CD148 may have a potential differential diagnostic value in leukemic B-CLPDs, especially between CLL and MCL.

Keywords::

• Diagnosis
• flow cytometry
• CD200
• CD148
• lymphoma

Acknowledgements

This study was supported by the National Natural Science Foundation of China (Nos. 81200360, 81100352, 81170485, 81170486, 81170488, 81370657), Natural Science Foundation of Jiangsu Province (No. BK2012484), Key Projects of Health Department of Jiangsu Province (No. K201108), Jiangsu Province's Medical Elite Program (No. RC2011169), National Public Health Grand Research Foundation (No. 201202017), Project funded by the Priority Academic Program Development of Jiangsu Higher Education Institute (No. JX10231801), Program for Development of Innovative Research Teams in the First Affiliated Hospital of Nanjing Medical University, Project of National Key Clinical Specialty, National Science and Technology Pillar Program (No. 2014BAI09B12), Project funded by Jiangsu Provincial Special Program of Medical Science (No. BL2014086), Jiangsu TePin Professor Program, Department of Education of Jiangsu Province, and Jiangsu TePin Medical Expert Program, Health and Family Planning Commission of Jiangsu Province.

Potential conflict of interest

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