Treatment of BCL-1 Murine B-Cell Leukemia with Recombinant Cytokines: Comparative Analysis of the Anti-Leukemic Potential of Interleukin 1 Beta (IL-1 β), Interleukin 2 (IL-2), Interleukin-6 (IL-6), Tumor Necrosis Factor Alpha (TNFα), Granulocyte Colony Stimulating Factor (G-CSF), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), and their Combination

Abstract

Murine BCL-1 B-cell leukemia provides a model of disseminated human B-lineage acute lymphoblastic leukemia (ALL) and non-Hodgkins lymphoma (NHL). This model was used to evaluate and compare the anti-leukemic efficacy of recombinant cytokines rIL-1β, rIL-2, rIL-6, rTNFα, rG-CSF, rGM-CSF and their combinations. Of these 6 cytokines tested, rG-CSF, rIL-1β, rIL-2, and rTNFα exerted a marked anti-leukemia/lymphoma activity, as reflected by significantly improved survival of treated mice after inoculation of BCL-1 cells. Notably, no additive or synergistic effects were observed when 2-4 cytokines were used in combination. To our knowledge, this report represents the first comparative analysis of recombinant cytokine treatment regimens in an animal model of disseminated B-lineage ALL/NHL.

Key Words:

• Immunotherapy
• BCL-1 leukemia
• cytokines
• IL-1
• IL-2
• IL-6
• TNF
• G-CSF
• GM-CSF