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Abstract
Kinetic data facilitates an approach to the biological behaviour of malignant lymphomas. In this study of 164 patients with a B-cell malignant lymphoma both cellular DNA and protein content were quantified by flow cytometry. The percentage of cells in the various phases of the cell-cycle (G1, S, G2 + M) were calculated and were analysed according to clinical and biological features for all cases and in each histological class. Statistical differences were observed for S-phase (p < 0.0001) and the mean protein content (p < 0.01) between the different histological groups, while no statistical difference was observed for G2 + M. Bone-marrow and blood infiltration was related to the percentage of cells in S or S + G2 + M phases (p < 0.005) in intermediate grade lymphomas. Amongst the biological variables such as LDH, β2 microglobulin or transferrin receptor the latter was the one which best related to cell proliferation (p < 0.001). Immunophenotype was also analysed and there was a correlation between the expression of surface antigens and cell proliferation, in particular, a negative correlation in the diffuse large cell group between CD24 expression and S-phase (p < 0.01), and a positive correlation between CD5 and S + G2 + M in immunoblastic lymphoma (p < 0.01). In this series, survival was significantly longer when S-phase index was less than 16% (p < 0.03) in aggressive lymphomas treated with intensive chemotherapy, and under 7.15% (p < 0.02) in other cases treated with non intensive chemotherapy.