c-myc Protein Expression During Cell Cycle Phases in Differentiating HL-60 Cells

Shunichi Kumakura Third Division of Internal Medicine and Division of Blood Transfusion, Shimane Medical University, Izumo, 693, Japan

Hiroto Ishikura Third Division of Internal Medicine and Division of Blood Transfusion, Shimane Medical University, Izumo, 693, Japan

Hiroto Tsumura Third Division of Internal Medicine and Division of Blood Transfusion, Shimane Medical University, Izumo, 693, Japan

Hitoshi Hayashi Third Division of Internal Medicine and Division of Blood Transfusion, Shimane Medical University, Izumo, 693, Japan

Jiro Endo Third Division of Internal Medicine and Division of Blood Transfusion, Shimane Medical University, Izumo, 693, Japan

Tokugoro Tsunematsu Third Division of Internal Medicine and Division of Blood Transfusion, Shimane Medical University, Izumo, 693, Japan

Abstract

We examined c-myc protein expression in cell cycle phases during differentiation induction of HL-60 cells by flow cytometry using an indirect immunofluorescence method. In exponentially proliferating HL-60 cells, c-myc protein was expressed in a cell cycle dependent manner. During the differentiation induction of HL-60 cells with dimethylsulfoxide (DMSO), c-myc protein was rapidly down-regulated in the G_1/0 specific phase prior to the appearance of differentiation associated markers. Our results indicate that c-myc protein functions in the G_1/0 specific phase in cellular differentiation, and the rapid down-regulation of c-myc protein in G^1/0 phase is closely associated with initial differentiation programs.

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c-myc Protein Expression During Cell Cycle Phases in Differentiating HL-60 Cells

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c-myc Protein Expression During Cell Cycle Phases in Differentiating HL-60 Cells

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