Expression of MDR-1/P-Glycoprotein in Childhood Acute Megakaryoblastic Leukemia Cells

Setsuo Ota Department of Pediatrics, School of Medicine, Chiba University, Chiba

Takeyuki Sato Department of Pediatrics, School of Medicine, Chiba University, Chiba

Harumi Kakuda Department of Pediatrics, School of Medicine, Chiba University, Chiba

Kiyomi Hirano Department of Pediatrics, School of Medicine, Chiba University, Chiba

Yuri Okimoto Division of Hematology and Oncology, Chiba Children's Hospital, Chiba

Taijiro Mori Department of Pediatrics, Saitama Central Hospital, Saitama

Shinpei Nakazawa Department of Pediatrics, Yamanashi Medical University, Yamanashi

Seiji Kojima Division of Hematology and Oncology, Children's Medical Center, Japanese Red Cross Nagoya First Hospital, Nagoya

Takaharu Matsuyama Division of Hematology and Oncology, Children's Medical Center, Japanese Red Cross Nagoya First Hospital, Nagoya

Takashi Tsuruo Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo

Akira Fuse Department of Safety Control on Biologics, National Institute of Health, Tokyo, Japan

Hiroo Niimi Department of Pediatrics, School of Medicine, Chiba University, Chiba

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Abstract

Multidrug resistance is a major clinical problem in chemotherapy of malignant disease. Acute megakaryoblastic leukemia (AMKL) is a rare form of childhood leukemia, and is often more resistant to many anticancer chemotherapeutic drugs compared to other types of childhood leukemia. There have been reports of the increased expression in hematologic malignancy of multidrug resistant (mdr-I) gene, which encodes for a transmembrane glycoprotein P-glycoprotein that acts as an efflux pump for structurally unrelated chemotherapeutic drugs. We investigated the malignant cells of 15 newly diagnosed childhood AMKL patients by immunocytochemical analysis and found P-glycoprotein expression in all samples from these patients. RNA prepared from five patients at the time of presentation confirmed the expression of mdr-I specific message in all cases by Northern blot analysis. These results imply that malignant cells from all childhood AMKL might express the mdr-I/P-glycoprotein.

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Expression of MDR-1/P-Glycoprotein in Childhood Acute Megakaryoblastic Leukemia Cells

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Expression of MDR-1/P-Glycoprotein in Childhood Acute Megakaryoblastic Leukemia Cells

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