Differentiating Agents Do Not Induce a True Hairy Cell Phenotype in B-CLL Cells in Vitro

Ron Gartenhaus From the Division of Hematology-Oncology, Department of Medicine, and the Department of Pathology, Long Island Jewish Medical Center, New Hyde Park, New York; Albert Einstein College of Medicine, Bronx, New YorkUSA

Mark Hoffman From the Division of Hematology-Oncology, Department of Medicine, and the Department of Pathology, Long Island Jewish Medical Center, New Hyde Park, New York; Albert Einstein College of Medicine, Bronx, New YorkUSA

Alex Fuchs From the Division of Hematology-Oncology, Department of Medicine, and the Department of Pathology, Long Island Jewish Medical Center, New Hyde Park, New York; Albert Einstein College of Medicine, Bronx, New YorkUSA

Henny Billet From the Division of Hematology-Oncology, Department of Medicine, and the Department of Pathology, Long Island Jewish Medical Center, New Hyde Park, New York; Albert Einstein College of Medicine, Bronx, New YorkUSA

Ping Wang From the Division of Hematology-Oncology, Department of Medicine, and the Department of Pathology, Long Island Jewish Medical Center, New Hyde Park, New York; Albert Einstein College of Medicine, Bronx, New YorkUSA

Pam Gentile From the Division of Hematology-Oncology, Department of Medicine, and the Department of Pathology, Long Island Jewish Medical Center, New Hyde Park, New York; Albert Einstein College of Medicine, Bronx, New YorkUSA

Kanti Rai From the Division of Hematology-Oncology, Department of Medicine, and the Department of Pathology, Long Island Jewish Medical Center, New Hyde Park, New York; Albert Einstein College of Medicine, Bronx, New YorkUSA

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Abstract

B-Chronic lymphocytic leukemia (B-CLL) and hairy cell leukemia (HCL) are both differentiated B-cell lymphoproliferative disorders. Prior studies have suggested that phorbol esters and the macro-cyclic lactone Bryostatin-1, which are both protein kinase-C activators, can induce the differentiation of B-CLL cells into HCL cells in vitro, as evidenced by morphology, phenotype and TRAP activity. The differentiating effect of all-trans retinoic acid on B-CLL cells has been less extensively studied. We studied the effects of incubating adherence purified B-CLL cells with phorbol myristic acetate (PMA), all-trans retinoic acid (ATRA), and Bryostatin-1. None of these agents induced a true HCL phenotype (CD5-, CD1 lc/CD25 coexpression) under the conditions studied.

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Differentiating Agents Do Not Induce a True Hairy Cell Phenotype in B-CLL Cells in Vitro

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Differentiating Agents Do Not Induce a True Hairy Cell Phenotype in B-CLL Cells in Vitro

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