Abortive Mitoses and Nuclear DNA Fragmentation in CD30+ Large Cells of Hodgkin's Disease

Lorenzo Leoncini Institute of Pathologic Anatomy and Histology, University of Siena, Italy; Department of Anatomical Pathology, University of Cape Town, South Africa

Donatella Spina Institute of Pathologic Anatomy and Histology, University of Siena, Italy; Department of Anatomical Pathology, University of Cape Town, South Africa

Pauline Close Unit of Hemolymphopathology, 2nd Service of Pathologic Anatomy, Institute of Hematology “L. & A. Serágnoli”, University of Bologna, Italy

Tiziana Megha Institute of Pathologic Anatomy and Histology, University of Siena, Italy; Department of Anatomical Pathology, University of Cape Town, South Africa

Lorenzo Pacenti Institute of Pathologic Anatomy and Histology, University of Siena, Italy; Department of Anatomical Pathology, University of Cape Town, South Africa

Piero Tosi Institute of Pathologic Anatomy and Histology, University of Siena, Italy

Stefano Pileri Unit of Hemolymphopathology, 2nd Service of Pathologic Anatomy, Institute of Hematology “L. & A. Serágnoli”, University of Bologna, Italy

Antonio De Vivo Unit of Hemolymphopathology, 2nd Service of Pathologic Anatomy, Institute of Hematology “L. & A. Serágnoli”, University of Bologna, Italy

Rainer Kraft Institute of Pathology, University of Berne, Switzerland

Jean A. Laissue Institute of Pathology, University of Berne, Switzerland

Hans Cottier Institute of Pathology, University of Berne, Switzerland

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Abstract

This study was undertaken to better comprehend the reasons for the scarcity of Hodgkin and Reed-Sternberg (H-RS) cells in Hodgkin's disease (HD) despite their expression of “proliferation-associated antigens”. To this end, we assessed the relative frequency of mitotic phases and nuclear damage (detected by in situ end-labeling of DNA strand breaks) in CD30⁺ large cells of nodular sclerosis and mixed cellularity HD. Our results show that a) most CD30⁺ cells in HD exhibit abortive mitoses, with a highly significant arrest at the metaphase-ana/telophase transition, and b) many of these elements, i.e. mainly H-RS cells, show fragmentation of nuclear DNA, suggesting imminent or actual death. Percentages of CD30⁺ cells that entered mitosis and those with DNA strand breaks were of a similar order of magnitude and correlated significantly in a linear fashion. These findings are consistent with the concept that cell deletion is the major cause of the paucity of H-RS cells in HD.

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Abortive Mitoses and Nuclear DNA Fragmentation in CD30+ Large Cells of Hodgkin's Disease

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Abortive Mitoses and Nuclear DNA Fragmentation in CD30+ Large Cells of Hodgkin's Disease

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