Resistance to Apoptosis Induced by Serum Depletion and All-Trans Retinoic Acid in Drug-Resistant Leukemic Cell Lines

Toshio Kakihara Department of Pediatrics, Niigata University School of Medicine, Niigataa, Japan

Takeaki Fukuda Second Department of Pathology, Fukushima Medical College, Hikarigaoka, Fukushima, Japan

Tomoko Kamishima Second Department of Pathology, Niigata University School of Medicine, Niigata, Japan

Makoto Naito Second Department of Pathology, Niigata University School of Medicine, Niigata, Japan

Atsushi Tanaka Department of Pediatrics, Niigata University School of Medicine, Niigataa, Japan

Makoto Uchiyama Department of Pediatrics, Niigata University School of Medicine, Niigataa, Japan

Kenji Kishi First Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan

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Abstract

The relation between resistance to anticancer drugs and resistance to apoptosis has been investigated in the human leukemic cell line(KY-821) and its drug-resistant sublines. Under serum depletion conditions, drug-resistant cell lines showed apoptotic resistance when compared with the parental cell line. Drug resistant cell lines also showed resistance to apoptosis when treated with all-trans retinoic acid. DNA fragmentation was low in drug resistant cell lines under both stimulations. Flowcytometry analysis did not show any alterations of the Fas antigen, p53, bcl-2 and c-myc protein expression toward inhibition of apoptotic response in drug-resistant sublines. These results indicate that drug-resistant leukemic cells still show resistance to apoptosis-inducing stimulation such as poor nutrition and differentiation-inducing agents.

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Resistance to Apoptosis Induced by Serum Depletion and All-Trans Retinoic Acid in Drug-Resistant Leukemic Cell Lines

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Resistance to Apoptosis Induced by Serum Depletion and All-Trans Retinoic Acid in Drug-Resistant Leukemic Cell Lines

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