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Abstract
A method is described whereby nerve cells and processes, neuroglia and microglia may be stained using colloidal silver solutions (argyrol, silvol, 10% and 20%).
Fresh, unfixed brain tissue is stained in bulk in argyrol or silvol, and then dehydrated, embedded in low viscosity nitrocellulose, and sectioned. Before reduction the sections are treated with gold chloride to replace the silver. Sections are reduced in a formalin hydroquinone solution, fixed in sodium thiosulfate, dehydrated, and mounted in euparal. A method is described for removing the nitrocellulose before mounting.
No variation in the method was found to be necessary for the various species tested (rat, guinea pig, rabbit, and dog).