![Sample our Medicine, Dentistry, Nursing & Allied Health journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/5944/TOC-Subject-Sample-2021-Medicine-Dentistry-Nursing-Allied-Health.jpg"})
Abstract
Much difficulty has been experienced in obtaining basic fuchsins satisfactory for the Feulgen technic. A method of purification employing sulfur dioxide is described which has been found to improve many unsatisfactory fuchsins. It is also shown that if the best grade of commercial pararosanilin base is used and proper precautions observed in converting it to the chloride or the acetate, a product can be obtained which gives excellent results in the Feulgen technic. The methods by which the base can be converted to the chloride or acetate are described in detail. Precautions are given for avoiding, in the conversion of the base to the dye salts, contamination of the product with the impurities which interfere with the staining. The use of the acetate is recommended because of its greater solubility.