Fluorescence Microscopy Applied to Entomology and Allied Fields

Abstract

Fluorescence is a phenomenon observable in many substances including a wide range of biological constituents. By use of ultraviolet illumination and the proper fluorescent dyes, when needed, many details of structure and physiological differentiation are made apparent which by illumination with visible light are obscure.

The fluorescence microscope is a valuable adjunct to the study of fluorescence in biological materials. This instrument is discussed from a practical standpoint. The simplifications in the instrument which do not impair its efficiency are indicated.

The use of fluorochromes is discussed and a list of the most important of these is given. Important technics with the fluorescence microscope, including intravital microscopy, fluorescent photomicrography, and microspectroscopy, are described.