Abstract
The use of nigrosin in a rapid but accurate method for the demonstration of cilia bands, vacuoles and cytoplasmic inclusions in infusorians is discussed. The general effects of the dye as well as its staining action are presented together with a brief explanation of the physics of the action. The technic proposed involves mixing a drop of infusion on the slide with a drop of 10% aqueous nigrosin; excess stain is drained off, the specimen dried and mounted.