The Demonstration of a new nerve-cell Organoid

Abstract

A method is described for the demonstration of a new nerve-cell organoid— the binary spheroid systems (Baker bodies). Zenker-formol or acetic-osmium-bichromate materials are postchromed at 37°C. and embedded in paraffin, sectioned and mounted in the ordinary manner. The sections are colored in a 70% alcohol solution of Sudan black B, rinsed in 70% alcohol, counter-stained in Mayer's carmalum and mounted in Farrant's medium. After examination the cover may be removed and the Sudan black extracted in 96% alcohol. The sections can then be restained by the azan method of Heidenhain, and other cytoplasmic inclusions can be correlated accurately to the functional states of the spheroid systems.