Combination of Certain Fluorane Derivative dyes with Bacterial cells at Different Hydrogen on Concentrations

Abstract

While acid dyes have not been widely used for staining bacteria, several suggestions for their use have been advanced (Conn and Holmes, 1926; Maneval, 1941). In general these procedures call for adding an acid to the stain to intensify the combination of the dye with the bacterial cell. McCalla and Clark (1941) have demonstrated greater adsorption of acid dyes at lower pH levels. Conn and Holmes (1926) list a number of dyes derived from fluorane and compare their relative acidic tendencies, color intensities, and other properties. In a study of the influence of the acidic properties of dyes on their combination with bacterial cells at various hydrogen-ion concentrations, the data herein reported were obtained.