Mounting Media for phase Microscope Specimens

Abstract

-Results with phase microscopy can often be improved by the proper selection of mounting media. Temporary mounts can first be made in liquid mixtures of water and glycerol, butyl carbitol and alpha-chloronaphthalene, alpha-chloronaphthalene and alpha-bromonaphthalene or alpha-bromonaphthalene with methylene iodide to determine what index liquid serves best to emphasize structures of primary interest. Once this has been determined, permanent mounts can be prepared by the substitution of a solid mounting medium that most closely approaches the refractive index of the liquid.