Preparative chromatography for obtaining pure samples of rosaniline, magenta II, and new fuchsine

Abstract

A simple low pressure liquid chromatographic method is reported that can separate the basic fuchsine homologues, rosaniline, magenta II and new fuchsine from an impure commercial dye. The chromatographic purity of the separated dyes is > 90%. All homologues were obtained in multi-milligram amounts per chromatographic run; precise yields depend on the composition of the starting material and potentially may be greater. This is a useful preparative procedure for generating chromatographically pure samples of basic fuchsine homologues, especially those that cannot be obtained in pure form by direct synthesis.

Key words::

• high performance liquid chromatography
• low pressure column chromatography
• magenta II
• new fuchsine
• rosaniline
• thin layer chromatography

Declaration of interest: The author reports no conflicts of interest, and alone was responsible for the content and writing of the paper.