Trypan Blue as a Nuclear Stain for Plant Material

Abstract

Although McWhorter¹ and later Rich² mentioned that trypan blue stained the nuclei of plant cells, their procedures were concerned with the demonstration of virus inclusions. The following method was developed and is presented with the emphasis on nuclear staining. The present author hopes that others will try it for comparison with the popular aceto-carmine and aceto-orcein methods.