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Abstract
Several samples of J.S.B. stain (Jaswant Singh and Bhattacharjee, 1944) solution 1 (polychrome methylene blue) were prepared with 3-8 hr for dichromate-acid oxidation and addition of varying quantities of Na2HPO4 buffer for pH adjustment. Storage under severe tropical conditions and periodical checks by staining Plasmodium cynomolgi smears revealed that staining solutions oxidized 6-7 hr with a final pH of 7.8 gave optimum results. Some precipitation of azures, due to heat after 5 mo, adversely affected the quality of staining solutions, while cooler storage conditions were most favorable. Spectrophotometric and chromatographic studies indicated that the J.S.B. solution 1 was composed of blue and purple components, corresponding to higher methylene azures with methylene blue and thionin with allied products respectively.