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Abstract
Young leaf tips are soaked in a saturated aqueous esculin (aesculine) solution at 10-12° C for 15 min to 24 hr and fixed in acetic-alcohol, 1:1. The materials are then stained in a mixture of 2% aceto-orcein and 12V HCl (9:1), 3-4 sec over a flame followed by 30 min or longer at 30° C and then smeared in 1% aceto-orcein. Preparations are made permanent by loosening the cover glass in tertiary butyl alcohol and mounting directly in Canada balsam.