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Abstract
A method utilizing Carbowax 400 as the solvent for oil red O and Sudan IV is presented. This solvent fulfills all the requirements of an efficient solvent for lipid staining postulated by Chiffelle and Putt (1951) and, in addition, has several other advantages making it more practical than propylene glycol and ethylene glycol: (a) Carbowax sections are stained 3 times as fast, (b) frozen sections are stained 5 times as fast, (c) the staining solution is more readily prepared, and (d) the intensity of staining is greater.